Effect of anti-miR-33 treatment on gene expression in non-human primate liver

In this study the effect of anti-miR-33 treatment on plasma and liver lipid profiles was examined in non-human primates. A significant increase in plasma HDL-C, and a significant decrease in plasma triglyceride levels were observed. African green monkeys on a Normal chow diet were treated with anti-miR-33 or control anti-miR for 4 weeks and were then switched to a High Carb/Med. Cholesterol diet and treated with anti-miR-33 or control anti-miR for 8 more weeks (n=6/group). Gene expression profiling was performed on liver biopsies obtained at -5 weeks (baseline), 4 weeks and 12 weeks.

本研究以非人灵长类动物为实验对象，考察了抗miR-33 (anti-miR-33) 治疗对血浆与肝脏脂质谱的影响。研究观察到血浆高密度脂蛋白胆固醇（HDL-C）水平显著升高，血浆甘油三酯水平显著降低。实验中，非洲绿猴先以普通饲料日粮饲养，同时分别施加抗miR-33或对照抗miR处理，持续4周；随后将其转换为高碳水/中等胆固醇日粮，继续施加对应处理8周，每组动物数量为6只（n=6/group）。分别在第-5周（基线期）、第4周及第12周采集肝脏活检样本，开展基因表达谱分析。