Single-cell RNA-seq reveals the transcriptional landscape and heterogeneity of aortic macrophages in murine atherosclerosis

We have applied single-cell RNA sequencing as an unbiased profiling strategy to interrogate and classify aortic macrophage heterogeneity at the single-cell level in atherosclerosis. We performed single-cell RNA sequencing of total aortic CD45+ cells extracted from the non-diseased (chow fed) and atherosclerotic (11 or 20 weeks of high-fat diet) aorta of low-density lipoprotein receptor-deficient (Ldlr-/-) mice.

本研究采用单细胞RNA测序（single-cell RNA sequencing）作为无偏表征策略，在单细胞水平上探究并分类动脉粥样硬化中的主动脉巨噬细胞异质性。我们从低密度脂蛋白受体缺陷（Ldlr-/-）小鼠的正常饮食（普通饲料喂养）及高脂饮食11或20周的动脉粥样硬化主动脉中，提取总CD45+细胞并开展单细胞RNA测序。