GNPS_GC_ProspecTER_Sponge_Fm_20250505_Yunhai Li

GC-MS profiling of fraction MeOH of 29 sponge extracts from Irish waters. Freeze-dried sponges extracted by MeOH/DCM (1:1, v/v) and then fractionated by RP-SPE (1 g C18 stationary phase) into 4 fractions: H2O, H2O/MeOH (1:1), MeOH, MeOH/DCM (1:1).

针对采自爱尔兰海域的29份海绵提取物的甲醇馏分开展气相色谱-质谱联用（Gas Chromatography-Mass Spectrometry, GC-MS）表征分析。实验过程为：先以甲醇/二氯甲烷（1:1，体积比）对冷冻干燥海绵样品进行提取，随后采用反相固相萃取（Reversed-Phase Solid Phase Extraction, RP-SPE，1 g C18固定相）将所得粗提物分为4个组分：纯水（H₂O）、纯水/甲醇（1:1）、甲醇（Methanol, MeOH）以及甲醇/二氯甲烷（Dichloromethane, DCM）（1:1）。