Mixture compatibility of Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV) with pesticides used in soybean

ABSTRACT: Anticarsia gemmatalis (Hübner: 1818) (Lepidoptera: Erebidae) is one of the main pests that affect soybean crops, causing defoliation. In the vegetative stages, defoliation occurs together with weeds, and in the reproductive stages with pathogens. In this sense, to maintain plant health, it is necessary to carry out the combined use of pesticides. Thus, this research determined the compatibility of the entomopathogenic virus AgMNPV with the main herbicides and fungicides used in soy at different times of the mixture. The artificial diet was immersed in the solutions of the pesticides and their mixtures and supplied to A. gemmatalis caterpillars, immediately and after one and two hours of mixing. The evaluation was performed by quantifying the number of dead caterpillars by mixing the AgMNPV virus with herbicides and fungicides, even after two hours of mixing if compatible. The observed scenarios showed a compatibility of the virus with the herbicides and fungicides, with mortality rates between 70 to 99% for A. gemmatalis.

摘要：银纹夜蛾（Anticarsia gemmatalis Hübner: 1818，鳞翅目: 夜蛾科）是危害大豆作物的主要害虫之一，可引发大豆叶片缺刻脱落。在大豆营养生长阶段，该虫导致的落叶常与杂草危害复合发生；而在生殖生长阶段，则与病原物侵染协同为害。有鉴于此，为保障大豆植株健康，需采用农药联合施用策略。为此，本研究测定了昆虫病原病毒AgMNPV与大豆生产中常用的主要除草剂、杀菌剂在不同混配时段的相容性。将人工饲料分别浸泡于各农药单剂及其混配溶液中，分别在混配即刻、混配1小时及2小时后饲喂银纹夜蛾幼虫。本研究通过计数AgMNPV与除草剂、杀菌剂混配后的银纹夜蛾幼虫死亡数开展效果评估，若混配体系相容，则即使在混配2小时后仍可进行检测。试验结果显示，该病毒与供试除草剂、杀菌剂均具有良好相容性，银纹夜蛾幼虫的死亡率可达70%~99%。