five

Mitochondrial COXI sequences and associated metazoan abundances in soils collected from the McMurdo Dry Valleys, Antarctica from 1995 to 2022

收藏
DataONE2023-04-12 更新2024-06-08 收录
下载链接:
https://search.dataone.org/view/https://pasta.lternet.edu/package/metadata/eml/knb-lter-mcm/269/1
下载链接
链接失效反馈
官方服务:
资源简介:
As part of an ongoing long-term sampling effort conducted by the McMurdo Dry Valleys Long Term Ecological Research (LTER) project, the top 10 cm of soil was collected from sampling sites across the McMurdo Dry Valleys region of Antarctica. A subset of these samples representing each valley and a differing disturbance legacy from the last glacial maximum were analyzed for this data package. Samples were collected between 1995 and 2022. In each sample, the abundances of three genera of nematodes (Scottnema, Eudorylaimus, and Plectus), tardigrades, and rotifers were calculated. Following metazoan extractions, individual Scottnema lindsayae were selected for mitochondrial Cytochrome c oxidase I (COX1) Sanger dideoxy sequencing. Sequencing resulted in 249 assembled sequences of 285 base pairs after alignment and trimming.

本数据集隶属于麦克默多干谷长期生态研究(Long Term Ecological Research, LTER)项目的持续性长期采样工作。研究团队从南极洲麦克默多干谷区域的各采样点采集了表层0至10厘米的土壤样品。本数据包选取覆盖各干谷、且携带末次冰盛期以来各异扰动遗留特征的部分样品进行分析。样品采集周期为1995年至2022年。针对每份样品,研究人员统计了3个属的线虫(Scottnema、Eudorylaimus及Plectus)、缓步动物与轮虫的丰度。完成后生动物提取后,研究人员选取单条Scottnema lindsayae个体进行线粒体细胞色素c氧化酶亚基I(Cytochrome c oxidase I, COX1)桑格双脱氧测序。经序列比对与修剪后,最终获得249条长度为285碱基对的拼接序列。
创建时间:
2023-04-12
二维码
社区交流群
二维码
科研交流群
商业服务