The genome-wide targeting of H3K18la in H1299 cells. The genome-wide targeting of H3K18la in H1299 cells

L-lactate was reported as a precursor that can label and stimulate histone lysine-N-L-lactylation (Kla), which represents a new epigenetic mark affecting gene expression directly via histone PTMs under conditions of high glycolysis, such as the Warburg effect. To investigate the genome-wide targeting of H3K18la, we performed ChIP-seq in H1299 cells using anti-H3K18la antibody. 50.6% of the H3K18la binding sites displayed enrichment close to -1kb promoter of genes. More importantly, our ChIP-seq data showed that H3K18la is enriched in many genes that related with replication processes, highlighted the importance of histone Kla involved in DNA replication. Overall design: Human NSCLC cell line H1299, including anti-H3K18la and IgG group.

已有研究表明，L-乳酸（L-lactate）可作为前体物质，标记并诱导组蛋白赖氨酸-N-乳酰化（Kla）；该修饰是一类新型表观遗传标记，在糖酵解水平较高的条件下（如瓦博格效应（Warburg effect）），可通过组蛋白翻译后修饰（post-translational modification, PTM）直接调控基因表达。为探究H3K18la的全基因组靶向分布特征，我们以H1299细胞为实验模型，使用抗H3K18la抗体开展染色质免疫沉淀测序（ChIP-seq）实验。分析结果显示，50.6%的H3K18la结合位点富集于基因转录起始位点上游约1kb的启动子区域。更为重要的是，本研究的ChIP-seq数据表明，H3K18la在大量与复制过程相关的基因中存在富集，凸显了组蛋白乳酰化参与DNA复制过程的关键作用。实验整体设计：采用人非小细胞肺癌（non-small cell lung cancer, NSCLC）细胞系H1299，设置抗H3K18la抗体实验组与免疫球蛋白G（IgG）对照组。