A general transcription co-factor, Smad3, potentiates master transcription factor mediated cell identity conversions [ATAC-seq]. Mus musculus

We have identified that expression of constitutive active Smad3 (Smad3CA) significantly enhanced reprogramming by Oct4, Sox2, Klf4, c-Myc. To investigate how Smad3CA expression impacts on chromatin opening, we performed ATAC-seq on day 3 of reprogramming in the presence or absence of Smad3CA expression. Overall design: ATAC-seq on day 3 of reprogramming in the presence or absence of Smad3CA expression.

我们已证实，组成型激活型Smad3（Smad3CA）的表达可显著增强Oct4、Sox2、Klf4、c-Myc介导的细胞重编程。为探究Smad3CA表达对染色质开放状态的调控机制，我们在重编程第3天分别对存在Smad3CA表达与不存在Smad3CA表达的样本进行了ATAC-seq测序。实验整体设计：在重编程第3天，分别对存在Smad3CA表达与不存在Smad3CA表达的细胞开展ATAC-seq测序。