Data_Sheet_1_Affinity of Serum Albumin and Fibrinogen to Cellulose, Its Hydrophobic Derivatives and Blends.docx

This work describes the preparation of spin-coated thin polymer films composed of cellulose (CE), ethyl cellulose (EC), and cellulose acetate (CA) in the form of bi- or mono-component coatings on sensors of a quartz crystal microbalance with dissipation monitoring (QCM-D). Depending on the composition and derivative, hydrophilicity can be varied resulting in materials with different surface properties. The surfaces of mono- and bi-component films were also analyzed by atomic force microscopy (AFM) and large differences in the morphologies were found comprising nano- to micrometer sized pores. Extended protein adsorption studies were performed by a QCM-D with 0.1 and 10 mg mL−1 bovine serum albumin (BSA) and 0.1 and 1 mg mL−1 fibrinogen from bovine plasma in phosphate buffered saline. Analysis of the mass of bound proteins was conducted by applying the Voigt model and a comparison was made with the Sauerbrey wet mass of the proteins for all films. The amount of deposited proteins could be influenced by the composition of the films. It is proposed that the observed effects can be exploited in biomaterial science and that they can be used to extent the applicability of bio-based polymer thin films composed of commercial cellulose derivatives.

本研究报道了一种薄膜制备方法：以纤维素（CE）、乙基纤维素（EC）和醋酸纤维素（CA）为原料，采用旋涂法制备双组分或单组分聚合物涂层，并将其涂覆于耗散型石英晶体微天平（Quartz Crystal Microbalance with Dissipation Monitoring, QCM-D）传感器表面。根据薄膜组分及衍生物种类的差异，可对其亲水性进行调控，从而获得具有不同表面特性的材料。采用原子力显微镜（Atomic Force Microscopy, AFM）对单组分及双组分薄膜的表面进行表征后发现，其表面形貌存在显著差异，包含纳米至微米级孔隙结构。进一步开展了扩展型蛋白质吸附实验：使用耗散型石英晶体微天平（QCM-D），在磷酸盐缓冲液体系中，分别以浓度为0.1 mg·mL⁻¹和10 mg·mL⁻¹的牛血清白蛋白（Bovine Serum Albumin, BSA），以及浓度为0.1 mg·mL⁻¹和1 mg·mL⁻¹的牛血浆纤维蛋白原作为吸附底物。采用沃伊特模型（Voigt Model）对结合蛋白的质量进行分析，并将所有薄膜的分析结果与对应蛋白质的绍尔布雷湿重（Sauerbrey Wet Mass）进行对比。实验结果表明，薄膜组分可对沉积蛋白的吸附量产生影响。本研究提出，上述观测到的效应可应用于生物材料科学领域，同时有望拓展以商用纤维素衍生物为原料制备的生物基聚合物薄膜的应用范围。