Primers used for preparation of CHIKV 181/25 clone expressing nLuc (CHIKV 181/25-nLuc) and for sequencing PCR fragment from cDNA of V5040 virus.

The nLuc gene was inserted within the nsP3 gene by Gibson cloning method at 5217 bp site as described in Materials and Methods.如材料与方法部分所述，采用Gibson克隆法（Gibson cloning）将nLuc基因（nLuc gene）插入至nsP3基因（nsP3 gene）内部的5217 bp（碱基对，base pair）位点处。