Multiomic Evaluation of Metastasis Evolution, from Primary, CTC, to Metastatic Tumors. Multiomic Evaluation of Metastasis Evolution, from Primary, CTC, to Metastatic Tumors

Metastasis is the most important cause of cancer-related death and it has been the most important issue both in basic and clinical cancer researches. Through in vivo spontaneous metastasis animal model, we successfully cultivated and expanded the metastasis lineage cells for genomic, methylomic, and transcriptomic analyses based on the high efficiency and excellent viability of a modified CTC-capturing system together with primary and metastasis tumors as well as parental 4T1-hHER2 breast cancer cell line. Overall design: By using in vivo spontaneous metastasis animal model from NSG mice to harvest all metastatic lineages of cancer cells from primary tumor, circulating tumor cell, and metastasis tumor for following evaluation on cancer metastasis evolution.

转移是癌症相关死亡的最主要诱因，同时也是基础癌症研究与临床癌症研究领域的核心议题。本研究借助体内自发转移动物模型，结合经改造的循环肿瘤细胞（CTC, Circulating Tumor Cell）捕获系统所具备的高效性与优异活性，同时以原发肿瘤、转移瘤及亲本4T1-hHER2乳腺癌细胞系为材料，成功培养并扩增了转移谱系细胞，用于基因组学、甲基化组学及转录组学分析。整体实验设计：本研究采用来自NSG免疫缺陷小鼠的体内自发转移动物模型，从原发肿瘤、循环肿瘤细胞及转移瘤中获取所有癌症细胞转移谱系，用于后续癌症转移演化过程的相关评估。