Resveratrol attenuates oxidative stress and muscle damage in sedentary rats subjected to physical exercise

ABSTRACT Physical inactivity is a public health problem when a sedentary population practices physical activity sporadically. Exercise in unconditioned individuals causes oxidative stress and muscle damage. This study tested the hypothesis that a single physical exertion can cause muscle damage and oxidative stress in sedentary individuals, and resveratrol can attenuate it. For this, 40 sedentary adult male rats were equally and randomized into four groups subjected to 90min swimming or rest and administered aqueous resveratrol (100mg/kg/day) or saline for 14 days: N-RV-, rats maintained at rest and administered saline; N-RV+, rats maintained at rest and treated with resveratrol; N+RV-, rats subjected to physical exercise and administered saline; and N+RV+, rats subjected to physical exercise and treated with resveratrol. In sedentary rats, the physical exertion of swimming promoted oxidative stress, i.e. increased lipid peroxidation and decreased plasma total antioxidant capacity, and significant increases in CK and LDH plasma activities. Resveratrol diminished lipid peroxidation and the concentrations of muscle damage markers (CK and LDH) in sedentary rats subjected to swimming. The results provide evidence that a single sudden physical exertion can cause oxidative stress in sedentary rats. Resveratrol showed good results as a treatment for minimizing muscle damage caused by this stress.

摘要：久坐人群若仅偶尔开展体力活动，运动不足仍是一项公共卫生难题。未经过运动适应的个体进行运动时，会引发氧化应激与肌肉损伤。本研究旨在验证如下假说：单次体力负荷可使久坐个体产生肌肉损伤与氧化应激，而白藜芦醇（resveratrol）可对此起到缓解作用。为此，本研究将40只成年雄性久坐大鼠按均等原则随机分为四组，使其分别接受90分钟游泳运动或静息处理，并连续14天给予水溶性白藜芦醇（100mg/kg/天）或生理盐水：N-RV-组：静息并给予生理盐水的大鼠；N-RV+组：静息并给予白藜芦醇的大鼠；N+RV-组：进行体力运动并给予生理盐水的大鼠；N+RV+组：进行体力运动并给予白藜芦醇的大鼠。在久坐大鼠中，游泳所致的体力负荷可诱发氧化应激，具体表现为脂质过氧化水平升高、血浆总抗氧化能力降低，同时血浆肌酸激酶（CK）与乳酸脱氢酶（LDH）活性显著升高。白藜芦醇可降低游泳久坐大鼠的脂质过氧化水平与肌肉损伤标志物（CK、LDH）浓度。本研究结果证实，单次突发性体力负荷可使久坐大鼠产生氧化应激。白藜芦醇在缓解此类应激所致肌肉损伤的治疗中表现出良好效果。