KSHV infection of endothelial cells induces actin dynamics and rearrangements of the actin cytoskeleton.

HUVEC were infected with KSHV and stained for actin cytoskeleton (green) and nuclei (blue) at different time points post-infection. Actin stress fibers (while arrow head) and cortical actin structures (white arrow) were visible before viral infection. KSHV infection disrupted actin stress fibers and induced more cortical actin structures at the early stage of infection (<15 mpi). However, cortical actin structures started to dissolve accompanying the reappearance of short and thick actin filaments resembling actin tails/spikes (red arrow head) after 15 mpi. Membrane ruffling, lamellipodia and filopodia were visible after 15 mpi (red arrow head).

本数据集以人脐静脉内皮细胞（Human Umbilical Vein Endothelial Cells, HUVEC）为研究对象，采用卡波西肉瘤相关疱疹病毒（Kaposi's Sarcoma-Associated Herpesvirus, KSHV）感染细胞，并在感染后的不同时间点对肌动蛋白细胞骨架（actin cytoskeleton）与细胞核分别进行绿色荧光染色与蓝色荧光染色。病毒感染前，即可观察到肌动蛋白应力纤维（actin stress fibers，以白色箭头头标注）与皮层肌动蛋白结构（cortical actin structures，以白色箭头标注）。KSHV感染可在感染早期（<15 mpi，minutes post-infection，即感染后分钟数）破坏肌动蛋白应力纤维，并诱导产生更多皮层肌动蛋白结构。然而在感染15分钟后（>15 mpi），皮层肌动蛋白结构开始解体，同时伴随出现形似肌动蛋白尾/棘突的短粗肌动蛋白丝，该结构以红色箭头头标注。感染15分钟后，还可观察到膜褶皱（membrane ruffling）、片状伪足（lamellipodia）与丝状伪足（filopodia），上述结构均以红色箭头头标注。