Time course RNA-seq analysis of barley powdery mildew fungus Bgh infecting immunocompromised Arabidopsis thaliana. Blumeria hordei

RNA-seq experiments detected fungal expression patterns during infection of immunocompromised Arabidopsis thaliana Overall design: 3 biological replicates per condition. Arabidopsis plants without (pps) or with (B12) the MLA1-HA construct in pen2 pad4 sag101 background were challenged with either Bgh isolate K1 expressing the cognate AVRA1 effector for MLA1 or Bgh isolate A6 expressing other AVRA effectors. Samples were collected at 6, 12, 18, 24 hours post inoculation (hpi) of Bgh.

本RNA测序（RNA-seq）实验旨在探究免疫缺陷型拟南芥（Arabidopsis thaliana）被真菌感染过程中的真菌基因表达模式。实验整体设计如下：每个处理条件设置3次生物学重复。在pen2 pad4 sag101遗传背景下，分别获得不含（pps）与携带（B12）MLA1-HA载体的拟南芥植株，随后分别用表达适配MLA1的同源AVRA1效应蛋白的Bgh菌株K1，以及表达其他AVRA效应蛋白的Bgh菌株A6进行接种处理。样本采集时间点为接种Bgh后的6、12、18和24小时（hpi）。