Genome and transcriptome sequencing of Acinetobacter baumannii strains AB5075-UW and ATCC17978-UN to tigecycline stress

Sequencing results associated with the manuscript titled: History shapes regulatory and evolutionary responses to tigecycline in strains of Acinetobacter baumannii from the pre- and post-antibiotic eras by Alecia B. Rokes, Alfonso Santos-Lopez, and Vaughn S. Cooper. This project assessed the transcriptomes of two strains of A. baumannii, AB5075-UW and ATCC17978UN, to tigecycline stress. Populations of both strains were evolved in increasing concentrations of tigecycline and whole-genome sequencing was performed to determine genetic adaptations of resistance. Lastly, tigecycline-evolved population transcriptome sequencing was done in the presence and absence of tigecycline stress.

本数据集关联Alecia B. Rokes、Alfonso Santos-Lopez与Vaughn S. Cooper发表的题为《历史塑造抗生素时代前后鲍曼不动杆菌（Acinetobacter baumannii）菌株对替加环素（tigecycline）的调控与进化应答》的研究论文。本研究针对两株鲍曼不动杆菌AB5075-UW和ATCC17978UN，分析其在替加环素胁迫下的转录组（transcriptome）；将两菌株的种群置于浓度逐步升高的替加环素环境中进行传代进化，并通过全基因组测序（whole-genome sequencing）鉴定其耐药性相关的遗传适应性变异；最后，对经替加环素驯化的种群，分别在有、无替加环素胁迫的条件下开展了转录组测序。