scRNA-seq of aged Drosophila midgut under methionine restriction

Methionine restriction is known to extend lifespan in various model organisms including Drosophila melanogaster. In this analysis, we performed scRNAseq of Drosophila female midgut samples to understand the cell type specific response to methionine restriction. The midgut samples were obtained from young (day5), aged (5 weeks old), or aged flies fed with methionine-restricted diet for four weeks. Methionine restriction was achieved by reducing 90% of dietary methionine using the chemically defined diet, which has 40% of all amino acids compared to the original recipe. This resulted in the 1.6 mM methionine in the control, which 0.16mM methionine in the restricted diet.

已知蛋氨酸限制（Methionine restriction）可延长包括黑腹果蝇（Drosophila melanogaster）在内的多种模式生物的寿命。本研究针对黑腹果蝇雌性中肠样本开展单细胞RNA测序（scRNAseq），以解析细胞类型特异性的蛋氨酸限制应答反应。本次实验的中肠样本来源于三类果蝇：5日龄年轻果蝇、5周龄老年对照果蝇，以及经4周蛋氨酸限制饮食处理的5周龄老年果蝇。本研究采用化学成分明确的合成培养基实现蛋氨酸限制：相较于基础配方，该培养基的总氨基酸含量为原配方的40%，并将日粮蛋氨酸水平降低90%。以此方式构建的对照组日粮蛋氨酸浓度为1.6 mM，而蛋氨酸限制组日粮的蛋氨酸浓度仅为0.16 mM。