Cointegration and cassette integration frequencies.
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aDonor strains also contain R388; pTRC99A.Kan did not expressed IntI1 (negative control); p112.Kan (a pTRC99A.Kan derivative) expressed IntI1. The pVeb, pVeb.aadB, pAttI.veb, pAttI.veb.aadB, pAttI.IS.veb, pAttI.IS.veb.aadB, pAttI.veb* and pAttI.veb*.aadB were used for recombination (Figure S1). pBBR1MCS.3, cloning vector (negative control).bThe limit of detection of our assay is −5.cStandard deviations calculated from three independent experiments.dND, Not determinable.eup to 484 CazR, RifR transconjugants were tested for tetracycline sensitivity.fIntegration frequency of the veb1 gene cassette alone.
创建时间：
2015-12-02
