Cointegration and cassette integration frequencies.

aDonor strains also contain R388; pTRC99A.Kan did not expressed IntI1 (negative control); p112.Kan (a pTRC99A.Kan derivative) expressed IntI1. The pVeb, pVeb.aadB, pAttI.veb, pAttI.veb.aadB, pAttI.IS.veb, pAttI.IS.veb.aadB, pAttI.veb* and pAttI.veb*.aadB were used for recombination (Figure S1). pBBR1MCS.3, cloning vector (negative control).bThe limit of detection of our assay is −5.cStandard deviations calculated from three independent experiments.dND, Not determinable.eup to 484 CazR, RifR transconjugants were tested for tetracycline sensitivity.fIntegration frequency of the veb1 gene cassette alone.a. 供体菌株同样携带R388质粒；pTRC99A.Kan未表达整合酶IntI1（Integrase IntI1），作为阴性对照；p112.Kan（pTRC99A.Kan的衍生质粒）可表达IntI1。用于重组实验的质粒包括pVeb、pVeb.aadB、pAttI.veb、pAttI.veb.aadB、pAttI.IS.veb、pAttI.IS.veb.aadB、pAttI.veb*及pAttI.veb*.aadB（见图S1）。pBBR1MCS.3为克隆载体，亦作为阴性对照。 b. 本检测方法的检出限为−5。 c. 标准差由三次独立实验计算得到。 d. ND：无法测定（Not determinable）。 e. 共对至多484株头孢他啶抗性（CazR）、利福平抗性（RifR）的接合子开展四环素敏感性检测。 f. 单独的veb1基因盒的整合频率。