Mutation detection with MutH, MutL, and MutS mismatch repair proteins.
收藏PubMed Central1996-04-30 更新2026-05-02 收录
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https://pmc.ncbi.nlm.nih.gov/articles/PMC39545/
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资源简介：
Escherichia coli methyl-directed mismatch repair is initiated by MutS-, MutL-, and ATP-dependent activation of MutH endonuclease, which cleaves at d(GATC) sites in the vicinity of a mismatch. This reaction provides an efficient method for detection of mismatches in heteroduplexes produced by hybridization of genetically distinct sequences after PCR amplification. Multiple examples of transition and transversion mutations, as well as one, two, and three nucleotide insertion/deletion mutants, have been detected in PCR heteroduplexes ranging in size from 400 bp to 2.5 kb. Background cleavage of homoduplexes is largely due to polymerase errors that occur during amplification, and the MutHLS reaction provides an estimate of the incidence of mutant sequences that arise during PCR. IMAGES:
提供机构：
National Academy of Sciences
创建时间：
1996-04-30
