Response of Saccharomyces cerevisiae to a rapid transfer from a fermentable to a nonfermentable carbon source.. Saccharomyces cerevisiae

The global gene expression program that accompanies the adaptation of Saccharomyces cerevisiae to an abrupt transfer from a fermentable to a nonfermentable carbon source was characterized by using a cDNA microarray to monitor the relative abundances and polysomal distributions of mRNAs. Features of the program included a transient reduction in global translational activity and a severe decrease in polysome size of transcripts encoding ribosomal proteins. A replicate experimental design type is where a series of replicates are performed to evaluate reproducibility or as a pilot study to determine the appropriate number of replicates for a subsequent experiments. Keywords: replicate_design Overall design: Using regression correlation

本研究利用cDNA微阵列（cDNA microarray）监测信使RNA（mRNA）的相对丰度与多聚核糖体（polysome）分布，对酿酒酵母（Saccharomyces cerevisiae）从可发酵碳源突然转移至非发酵碳源的适应过程中伴随的全局基因表达程序进行了表征。该程序的特征包括全局翻译活性的暂时性下降，以及编码核糖体蛋白的转录本的多聚核糖体尺寸显著减小。重复实验设计类型指通过开展一系列重复实验以评估实验可重复性，或作为预实验来确定后续实验所需的合适重复次数。关键词：replicate_design；整体实验设计：采用回归相关性分析