GNPS - Metabolites detected in the spent supernatant after growth of salmon gut-derived bacteria on chitin and mannotetraose

Bacteria were grown on a minimal medium supplemented with chitin or mannotetraose as a sole carbon source. Cell pellet was removed and supernatant analysed for untargeted metabolomics. The sample was prepared by pooling small equal aliquots from each sample, to create a representative average of the entire set.

将细菌接种于以几丁质（chitin）或甘露四糖（mannotetraose）作为唯一碳源的补充型基础培养基中培养。培养结束后，离心去除细胞沉淀，对所得上清液进行非靶向代谢组学（untargeted metabolomics）分析。样本制备方式为：从每份样本中取等量的小体积等分试样进行混合，以获得能够代表整体样本集的代表性平均样本。