Transcriptomic analysis of human laryngeal carcinoma cell line LIU-LSC-1 with depletion of Raptor. Transcriptomic analysis of human laryngeal carcinoma cell line LIU-LSC-1 with depletion of Raptor

Recent studies have shown that mTOR signalling have a promotive effect on cancer. In our clinical work, we found that mTOR signalling is commonly activated in laryngeal cancer tissues and influence the metabolism of cancer cells. To investigate how mTORC1 exerts these functions and its downstream regulatory genes, we knocked down Raptor in the laryngeal cancer cell line LIU-LSC-1 and sequenced the transcriptome of wild-type and Raptor knockdown cells. Overall design: Retinal mRNA profiles of wild type (WT) and shRaptor LIU-LSC-1 cells

已有研究证实，哺乳动物雷帕霉素靶蛋白（mTOR）信号通路对肿瘤具有促癌效应。在本临床研究中，我们观察到mTOR信号通路在喉癌组织中普遍激活，并可影响肿瘤细胞的代谢过程。为探究mTOR复合物1（mTORC1）介导上述功能的具体机制及其下游调控基因，我们在喉癌细胞系LIU-LSC-1中敲低了Raptor基因，并对野生型与Raptor敲低细胞的转录组进行了测序。实验设计：野生型（WT）与shRaptor转染的LIU-LSC-1细胞的视网膜mRNA表达谱