Thermal optimized PCR coupled to CRISPR-Cas12a for rapid detection of blaOXA-1 resistance gene

Supplementary files for the publication "Thermal optimized PCR coupled to CRISPR-Cas12a for rapid detection of blaOXA-1 resistance gene"
FASTA files of blaOXA sequences used for the crRNA design.

Supplementary File S1. Multiple sequence alignment of the DNA sequences retrieved from the CARD and NDARO databases related to the blaOXA-1 gene.
Supplementary File S2. Multiple sequence alignments of the DNA sequences retrieved from the CARD and NDARO databases related to the non-blaOXA-1 groups.

论文《热循环优化聚合酶链式反应（Polymerase Chain Reaction, PCR）偶联成簇规律间隔短回文重复序列相关蛋白12a（Clustered Regularly Interspaced Short Palindromic Repeats-associated protein 12a, CRISPR-Cas12a）快速检测β-内酰胺酶OXA-1（blaOXA-1）耐药基因》的补充文件
用于CRISPR RNA（crRNA）设计的blaOXA序列FASTA格式（FASTA format）文件。
补充文件S1：从综合抗生素抗性数据库（Comprehensive Antibiotic Resistance Database, CARD）与美国国家耐药菌数据库（National Database of Antibiotic Resistant Organisms, NDARO）中获取的与blaOXA-1基因相关的DNA序列多重比对结果。
补充文件S2：从综合抗生素抗性数据库（CARD）与美国国家耐药菌数据库（NDARO）中获取的与非blaOXA-1类群相关的DNA序列多重比对结果。