Gene expression based analysis of CroV infection of Cafeteria roenbergensis. Gene expression based analysis of CroV infection of Cafeteria roenbergensis

In this study, total RNA was extracted from cultures of Cafeteria infected with the Cafeteria roenbergensis virus BV-PW1 (CroV) and hybridised to a CroV microarray in order to determine the temporal expression profile during infection. Overall design: A straightforward measure of whether each spot was on or off was determined as the infection progressed starting at 0 h post infection (control), then 1, 2, 3, 6, 12, 24, 48 and 72 h pi. Oligonucleotides 50-70 bp in length were designed for 452 predicted CroV ORFs and printed onto amino silane treated glass slides.

本研究从感染了罗伦岑食堂藻病毒BV-PW1（Cafeteria roenbergensis virus BV-PW1，简称CroV）的宿主细胞培养物中提取总RNA，并与CroV基因芯片（microarray）进行杂交，以解析病毒感染过程中的时序表达谱。
实验设计：本研究以感染后0小时（对照组，0 h pi）为起始时间点，依次在感染后1、2、3、6、12、24、48及72小时（pi，即post infection，感染后）监测感染进程中每个芯片探针点的表达开闭状态，以此作为直观的量化判定依据。本研究针对452个预测的CroV开放阅读框（Open Reading Frame，ORF）设计了长度为50~70 bp的寡核苷酸探针，并将其点印至经氨基硅烷修饰的玻璃玻片上。