Cut and run analysis in human trophoblast stem cells (CT27) upon pulling down with GATA2 and GATA3 antibody. Cut and run analysis in human trophoblast stem cells (CT27) upon pulling down with GATA2 and GATA3 antibody

GATA transcription factors, particularly GATA2 and GATA3 are selectively expressed in the extraembryonic trophoblast lineage and regulates gene expression to promote trophoblast self-renewal during mammalian development. However, we have a poor understanding about the contribution of these GATA factors, in the process of syncytiotrophoblast (SynT) development. Thus, the goal of this study is to define the importance of GATA2 and GATA3 in orchestrating a global gene expression program that poises and commits mammalian trophoblast stem cells to the SynT lineage. Using the cut and run technique for GATA2 and GATA3 antibodies in the human trophoblast stem cells we are aiming to identify the direct targets of the GATA transcription factors that are essential for determining the SynT lineage. Three individual experiments were performed. Overall design: Cut&Run experiments using GATA2 and GATA3 antibodies in human trophoblast stem cells.

GATA转录因子（GATA transcription factors），尤其是GATA2与GATA3，选择性表达于胚外滋养层谱系，并在哺乳动物发育进程中调控基因表达以促进滋养层自我更新。然而，目前学界对于此类GATA转录因子在合体滋养层细胞（syncytiotrophoblast，SynT）发育过程中的具体贡献仍知之甚少。为此，本研究旨在明确GATA2与GATA3在调控全局性基因表达程序中的关键作用——该程序可使哺乳动物滋养层干细胞做好分化准备并定向分化为SynT谱系。本研究采用切割与释放（cut and run，CUT&RUN）技术，针对人滋养层干细胞中的GATA2与GATA3抗体开展实验，以期鉴定出GATA转录因子的直接靶基因，此类靶基因对于确定SynT谱系至关重要。本研究共完成三次独立重复实验。整体实验设计：在人滋养层干细胞中使用GATA2与GATA3抗体开展CUT&RUN实验。