LncRNA HIF1A-AS1 regulates the apoptosis and proliferation of vascular endothelial cells through globally regulating miRNAs expression [miRNA-Seq]. LncRNA HIF1A-AS1 regulates the apoptosis and proliferation of vascular endothelial cells through globally regulating miRNAs expression [miRNA-Seq]

In the current study, on the basis of establishment of PA-induced damage model of HUVECs, the silencing and overexpression (OE) of HIF1A-AS1 were implemented in the HUVECs, and the effects on cell viability, apoptosis, migration and invasion were explored, respectively. Functionally, the results indicated silencing of HIF1A-AS1 promoted the proliferation migration and invasion, and reduced the apoptosis of HUVECs. In contrast, OE of HIF1A-AS1 reduced proliferation transwell and invasion, promoted the apoptosis, suggesting that HIF1A-AS1 play vital roles in regulating HUVECs. Moreover, miRNA sequencing (miRNA-seq) and RNA sequencing (RNA-seq) were carried out, and the results indicated that HIF1A-AS1 globally mediate expression of miRNA and mRNA. The multiple target genes of DEmiRNA were associated with cell proliferation and apoptosis, and overlapped with the DEGs from RNA-seq, suggestion that HIF1A-AS1 might regulate the proliferation and apoptosis of HUVECs by regulating miRNA expression. Overall design: Examination the silencing and overexpression of HIF1A-AS1 in HUVECs

本研究在构建PA诱导的人脐静脉内皮细胞（Human Umbilical Vein Endothelial Cells，HUVECs）损伤模型的基础上，对HUVECs中HIF1A-AS1进行沉默与过表达（OE）操作，并分别探究其对细胞活力、凋亡、迁移及侵袭能力的影响。功能实验结果显示，沉默HIF1A-AS1可促进人脐静脉内皮细胞的增殖、迁移与侵袭，并抑制其凋亡；反之，过表达HIF1A-AS1则会降低细胞增殖、穿膜迁移与侵袭能力，同时促进细胞凋亡，表明HIF1A-AS1在调控人脐静脉内皮细胞功能中发挥关键作用。此外，本研究开展了微小RNA测序（miRNA sequencing，miRNA-seq）与RNA测序（RNA sequencing，RNA-seq），结果证实HIF1A-AS1可全局性调控微小RNA与信使RNA（mRNA）的表达水平。差异表达微小RNA（differentially expressed miRNA，DEmiRNA）的多个靶基因与细胞增殖及凋亡过程密切相关，且与RNA测序得到的差异表达基因（differentially expressed genes，DEGs）存在重叠，提示HIF1A-AS1可能通过调控微小RNA的表达，进而影响人脐静脉内皮细胞的增殖与凋亡过程。本研究整体实验设计为：考察HIF1A-AS1在人脐静脉内皮细胞中的沉默与过表达效应。