TMBIM6 deficiency leads to bone loss by accelerating osteoclastogenesis. TMBIM6 deficiency leads to bone loss by accelerating osteoclastogenesis

To screen for altered gene expression during osteoclastogenesis, BMM cells depleted of TMBIM6 with or without RANKL were subjected to gene expression profiling. Overall design: Transcriptome profiles of RANKL-treated BMMs, which were depleted of TMBIM6, were generated in duplicate using Hiseq2500.

为筛选破骨细胞生成（osteoclastogenesis）过程中的差异基因表达，我们对敲低TMBIM6的骨髓来源巨噬细胞（Bone Marrow-derived Macrophages, BMM）分别施加或不施加核因子κB受体活化因子配体（Receptor Activator of Nuclear Factor κB Ligand, RANKL）处理，随后进行基因表达谱分析。
整体实验设计：采用Hiseq2500测序平台，对经RANKL处理且敲低TMBIM6的BMM细胞生成转录组谱，实验设置两次生物学重复。