Thermal tolerance of cathelicidin-RC1.

Thermal tolerance of cathelicidin-RC1 was determined by measuring MICs after incubation at different temperatures. Cathelicidin-RC1 solution (2 mg/ml, dissolved in sterile deionized water) was incubated at different temperatures (4, 20, 37, 50, 70, and 90°C) for 1 h, then the MICs of the peptides against E. coli ATCC25922 were determined. The results represent mean values of three independent experiments performed in duplicates.

本研究通过对cathelicidin-RC1进行不同温度孵育后测定其最小抑菌浓度（Minimum Inhibitory Concentration，MIC），以明确其热耐受性。将以无菌去离子水溶解的2 mg/mL cathelicidin-RC1溶液，分别置于4、20、37、50、70及90℃条件下孵育1小时，随后测定该多肽对大肠埃希菌（Escherichia coli，E. coli）ATCC25922的最小抑菌浓度。本研究结果为三次独立重复实验（每组设置双平行样本）的均值。