Expression Profiling: Guanylate Cyclase Soluble Subunit Beta-3 (GUCY1B3) overexpression U87 MG cells. Homo sapiens

Total RNA were extracted from Guanylate Cyclase Soluble Subunit Beta-3 (GUCY1B3) overexpression U87 MG stable cell lines and U87 MG cells. Three RNA samples of each of the two cell lines were used for microarray analysis to compare gene expression profile Overall design: Guanylate Cyclase Soluble Subunit Beta-3 (GUCY1B3) was cloned into pCDNA3.1D/V5-His-TOPO plasmid (Invitrgen) and then transfected into U87 MG (ATCC HTB-1) cells to generate stable overexpression cells. RNA from the stable cell lines and U87 MG were used for microarray

本研究从可溶性鸟苷酸环化酶亚基β3（Guanylate Cyclase Soluble Subunit Beta-3，GUCY1B3）过表达U87 MG稳定细胞系及野生型U87 MG细胞中提取总RNA。两种细胞系各设置3份RNA样本，用于微阵列分析以比较二者的基因表达谱。
实验整体设计：将GUCY1B3克隆至pCDNA3.1D/V5-His-TOPO质粒（Invitrogen），随后转染U87 MG（ATCC HTB-1）细胞以构建稳定过表达细胞系。收集上述稳定过表达细胞系及野生型U87 MG细胞的RNA，用于微阵列实验。