Identification of competing endogenous RNA (ceRNA) and micro-RNA profiles and regulatory networks in 4-nonylphenol-induced impairment of sertoli cells II. Identification of competing endogenous RNA (ceRNA) and micro-RNA profiles and regulatory networks in 4-nonylphenol-induced impairment of sertoli cells II

Purpose: To uncover the mechanism of 4-nonylphenol-induced impairment of sertoli cells, we used RNA sequencing technology to compare the small RNA expression differences between the control groups and the 4-nonylphenol treated groups. Sertoli cells small RNA profiles of the control groups and the 4-nonylphenol treated groups were generated by deep sequencing, in triplicate. The library quality was determined using a Bioanalyzer 2100 (Agilent). The Illumina BGISEQ platform was used for small RNA sequencing. The quality of RNA-seq reads was examined using FastQC. Overall design: 4-nonylphenol-treated and control sertoli cells small RNA profiles of 24h were generated by deep sequencing, in triplicate.

研究目的：为揭示4-壬基酚（4-nonylphenol）诱导支持细胞（Sertoli cells）损伤的分子机制，本研究采用RNA测序技术，对比对照组与4-壬基酚处理组的小RNA表达差异。本研究通过深度测序，以三次生物学重复构建对照组与4-壬基酚处理组支持细胞的小RNA表达谱。文库质量采用安捷伦（Agilent）Bioanalyzer 2100生物分析仪进行检测。小RNA测序依托Illumina BGISEQ测序平台完成。使用FastQC工具对RNA测序读段的质量进行质控评估。实验整体设计：本研究通过深度测序，以三次生物学重复构建经4-壬基酚处理24小时的支持细胞及同期对照组支持细胞的小RNA表达谱。