Microscopy data: interaction of the gene vamp2 with RNA polymerase II clusters during early zebrafish embryo development

Microscopy image data containing fluorescently labeled gene loci, recruited RNA polymerase II, and elongating RNA polymerase II. This data set is for the gene <em>vamp2</em> and is obtained from fixed zebrafish embryos, collected at the developmental stages oblong, sphere, dome, 30% epiboly, and 50% epiboly (indicated in the file names). Data were recorded using an instant-SIM microscope (iSIM, VisiTech UK) with a 100X TIRF oil immersion objective (Nikon, NA 1.49, CFI SR HP Apo TIRF 100XAC Oil). Two independent experiments were performed (IF1, IF2 in the file name), for each experiment, between one and three samples were prepared per stage and experiment (001, 002, 003 in the file name). The image data are in the ND2 format (Nikon proprietary) and can be imported using the BioFormats importer (Open Microscopy Environment).本数据集为显微图像数据，包含荧光标记的基因位点、招募态RNA聚合酶II（RNA polymerase II）以及延伸态RNA聚合酶II（RNA polymerase II）。本数据集针对基因vamp2（vamp2），取材自固定后的斑马鱼胚胎，采集阶段涵盖长圆形期（oblong）、球形期（sphere）、穹顶期（dome）、30%外包期（30% epiboly）及50%外包期（50% epiboly），上述发育阶段信息均标注于文件名中。数据采集使用英国VisiTech公司生产的即时结构光照明显微镜（instant-SIM，iSIM），搭配100×总内反射荧光（Total Internal Reflection Fluorescence, TIRF）油浸物镜（尼康，数值孔径NA 1.49，型号为CFI SR HP Apo TIRF 100XAC Oil）。实验分为两组独立重复（文件名中以IF1、IF2标注）；针对每组实验的每个发育阶段，均制备1至3份样本，样本编号001、002、003标注于文件名中。图像数据采用尼康专有ND2格式存储，可通过开放显微镜环境（Open Microscopy Environment）提供的BioFormats导入工具进行读取。