C42B RNA-seq

We performed Hi-C, Micro-C, and capture Micro-C in human prostate cancer cells and compared chromatin interactions called using different methods. By integrating Micro-C with NOMe-seq, ChIP-seq, and RNA-seq, we investigated the relationships among nucleosome positioning of regulatory elements, chromatin interactions, and transcription. This work provides a framework for understanding the chromatin interactions among regulatory elements, nucleosome-depleted regions, and transcription. RNA-seq in C42B prostate cancer cells

我们在人类前列腺癌细胞中开展了Hi-C、Micro-C以及捕获型Micro-C实验，并对不同方法鉴定得到的染色质相互作用进行了比较分析。通过将Micro-C与NOMe-seq、染色质免疫共沉淀测序（ChIP-seq）以及RNA测序（RNA-seq）相结合，我们探究了调控元件的核小体定位、染色质相互作用与转录过程三者之间的关联。本研究为解析调控元件、核小体缺失区域与转录过程之间的染色质相互作用提供了研究框架。本数据集包含C42B前列腺癌细胞中的RNA测序数据。