Genome-wide maps of chromatin state in thymocytes from Nsd2-edited mice. Genome-wide maps of chromatin state in thymocytes from Nsd2-edited mice

We performed high-throughput profiling of histone modifications in mouse thymocytes in which Nsd2 were deleted (KO) or Nsd2 were substituted as following c.2717C>T; p.Pro906Leu that is an orthologous substitution of human NSD2 c.2714C>T; p.Pro905Leu (KI). We found that lysine 36 dimethylation were generally decreased in KO and KI. These were associated with regulation of gene expression. Overall design: Examination of histone H3 lysine 36 dimethylation in mice thymocytes.

本研究对两类基因修饰的小鼠胸腺细胞开展了组蛋白修饰的高通量谱分析：其一为Nsd2基因敲除（Knockout, KO）细胞，其二为携带c.2717C>T; p.Pro906Leu突变的敲入（Knock-in, KI）细胞——该突变是人类NSD2基因c.2714C>T; p.Pro905Leu的同源置换突变。研究发现，在KO与KI细胞中，组蛋白H3赖氨酸36二甲基化水平普遍降低。上述变化与基因表达调控存在关联。整体实验设计：对小鼠胸腺细胞中的组蛋白H3赖氨酸36二甲基化水平进行检测。