Functional Genomic Analyses of Hep-G2 Cell Line after FTO knockdown

To explore the molecular mechanism underlying glucose regulation by hepatic FTO, we used the human hepatocyte Hep-G2 cell line as an experimental platform and analyzed transcriptome changes following FTO knock-down. Overall design: Examination of three Hep-G2 cell lines: FTO is knocked down more efficiently in sh7 and less efficiently in sh2. The control is the scramble.

为探究肝脏FTO调控葡萄糖的分子机制，我们采用人肝细胞Hep-G2细胞系作为实验平台，对FTO敲低后的转录组变化进行了分析。实验总体设计：共设置三组Hep-G2细胞样本，其中sh7组的FTO敲低效率更高，sh2组的敲低效率较低，对照组为scramble对照。