Crystal structure of the core streptavidin mutant V21 (Y22S/N23D/S27D/Y83S/R84K/E101D/R103K/E116N) complexed with iminobiotin long tail (IMNtail) at 1.2 A resolution

Crystal structure of the core streptavidin mutant V21 (Y22S/N23D/S27D/Y83S/R84K/E101D/R103K/E116N) complexed with iminobiotin long tail (IMNtail) at 1.2 A resolution Descriptor: 6-({5-[(2E,3aS,4S,6aR)-2-iminohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoyl}amino)hexanoic acid, GLYCEROL, Streptavidin Authors: Kawato, T, Mizohata, E, Shimizu, Y, Meshizuka, T, Yamamoto, T, Takasu, N, Matsuoka, M, Matsumura, H, Tsumoto, K, Kodama, T, Kanai, M, Doi, H, Inoue, T, Sugiyama, A. Deposit date: 2014-10-01 Release date: 2015-02-18 Last modified: 2023-11-08 Method: X-RAY DIFFRACTION (1.2 Å) Cite: Structure-based design of a streptavidin mutant specific for an artificial biotin analogue. J.Biochem., 157, 2015

分辨率1.2埃（Å）的核心链霉亲和素（Streptavidin）突变体V21（Y22S/N23D/S27D/Y83S/R84K/E101D/R103K/E116N）与长尾亚胺生物素（iminobiotin long tail, IMNtail）复合物的晶体结构
描述项：6-({5-[(2E,3aS,4S,6aR)-2-亚氨基六氢-1H-噻吩并[3,4-d]咪唑-4-基]戊酰基}氨基)己酸、甘油、链霉亲和素（Streptavidin）
作者：Kawato, T、Mizohata, E、Shimizu, Y、Meshizuka, T、Yamamoto, T、Takasu, N、Matsuoka, M、Matsumura, H、Tsumoto, K、Kodama, T、Kanai, M、Doi, H、Inoue, T、Sugiyama, A.
存放日期：2014-10-01
发布日期：2015-02-18
最后修改日期：2023-11-08
实验方法：X射线衍射（X-RAY DIFFRACTION，1.2 Å）
引用文献：针对人工生物素类似物特异性链霉亲和素突变体的基于结构设计（Structure-based design of a streptavidin mutant specific for an artificial biotin analogue.），《生物化学杂志》（J.Biochem.），第157卷，2015年