AtGenExpress: Effect of ABA during seed imbibition

AtGenExpress: A multinational coordinated effort to uncover the transcriptome of the multicellular model organism Arabidopsis thaliana The activity of genes and their encoded products can be regulated in several ways, but transcription is the primary level, since all other modes of regulation (RNA splicing, RNA and protein stability, etc.) are dependent on a gene being transcribed in the first place. The importance of transcriptional regulation has been underscored by the recent flood of global expression analyses, which have confirmed that transcriptional co-regulation of genes that act together is the norm, not the exception. Moreover, many studies suggest that evolutionary change is driven in large part by modifications of transcriptional programs. An essential first step toward deciphering the transcriptional code is to determine the expression pattern of all genes. With this goal in mind, an international effort to develop a gene expression atlas of Arabidopsis has been underway since fall 2003. This project, dubbed AtGenExpress, is funded by the DFG, and will provide the Arabidopsis community with access to a large set of Affymetrix microarray data. As part of this collaboration, we have generated expression data from 80 biologicaly different samples in triplicate. This experiment studies the effect of two concentrations of ABA during seed imbibition in the Col-0 ecotype. Experimenter name = Eiji Nambara , Kazumi Nakabayashi , Yuji Kamiya Experimenter institute = AtGenExpress Keywords: Effect of ABA 8 samples were used in this experiment

AtGenExpress：一项跨国协作项目，旨在解析多细胞模式生物拟南芥（Arabidopsis thaliana）的转录组（transcriptome）。基因及其编码产物的活性可通过多种方式调控，而转录是核心调控层级——因为所有其他调控模式（如RNA剪接、RNA与蛋白质稳定性调控等）均依赖于基因先完成转录。近年来大量涌现的全球基因表达谱分析研究进一步凸显了转录调控的重要性，这些研究证实，协同发挥功能的基因的转录共调控是普遍现象而非特例。此外，多项研究表明，演化变化在很大程度上由转录程序的修饰所驱动。解析转录调控代码的关键第一步，便是确定所有基因的表达模式。基于这一目标，自2003年秋季起，一项构建拟南芥基因表达图谱的国际合作项目正式启动。该项目命名为AtGenExpress，由DFG资助，将为拟南芥研究共同体提供大量Affymetrix微阵列芯片数据。作为该合作项目的一部分，我们已针对80种不同生物学样本完成了三次生物学重复实验。本实验探究了哥伦比亚生态型（Col-0）中，脱落酸（ABA）在种子吸胀过程中的两种浓度效应。实验人员：Eiji Nambara、Kazumi Nakabayashi、Yuji Kamiya；实验机构：AtGenExpress；关键词：ABA效应；本实验共使用8个样本。