Data from: Signatures of north-eastern expansion and multiple refugia: Genomic phylogeography of the Pine Barrens Treefrog, Hyla andersonii (Anura: Hylidae)

Range fragmentation poses challenges for species persistence over time and may be caused by both historical and contemporary processes. We combined genomic data, phylogeographic model testing, and paleoclimate niche modeling to infer the evolutionary history of the Pine Barrens Treefrog (Hyla andersonii), a seepage bog specialist, in eastern North America to better understand the historical context of its fragmented distribution. We sampled H. andersonii populations across the three disjunct regions of the species’ range: Alabama/Florida (AF), the Carolinas (CL), and New Jersey (NJ). Phylogenetic relationships within H. andersonii were consistent between the nuclear species tree and mitochondrial analyses, indicating divergence between AF and CL/NJ (Atlantic clade) ~0.9 million years ago and divergence of the NJ clade ~0.15 mya. Several predictions of northeastern expansion along the Atlantic coast were supported by phylogeographic analyses. Model testing using genome-wide SNP data and species distribution models both provided evidence for multiple disjunct refugia. This comprehensive phylogeographic study of H. andersonii demonstrates a long history of range fragmentation within an endemic coastal plain species and highlights the influence of historical climate change on the current distribution of species and their genetic diversity.

分布范围破碎化会长期对物种存续构成挑战，其成因可涵盖历史与当代两类过程。我们整合基因组数据、系统发生生物地理学模型检验以及古气候生态位建模，以推断北美东部渗流沼泽特化物种——松沼雨滨蛙（Hyla andersonii）的演化历史，从而更好地理解其破碎化分布的历史背景。我们在该物种分布的三个隔离分布区开展采样：阿拉巴马州/佛罗里达州（AF）、卡罗来纳州群（CL）以及新泽西州（NJ）。核基因物种树与线粒体基因组分析所得的H. andersonii种内系统发生关系一致，表明AF支系与CL/NJ支系（大西洋支系）的分化时间约为90万年前，而NJ支系的分化时间约为15万年前。多项关于大西洋沿岸东北扩张的预测得到了系统发生生物地理学分析的支持。基于全基因组单核苷酸多态性（SNP）数据的模型检验与物种分布模型均为多隔离避难所假说提供了证据。这项针对H. andersonii的综合性系统发生生物地理学研究表明，这种特有海岸平原物种长期存在分布范围破碎化的历史，并凸显了历史气候变化对物种当前分布及其遗传多样性的影响。