Transcription profiling of Drosophila melanogaster SSDP target genes

Transcription profiling of third instar male larvae of two different heteroallelic combinations of ssdp hypomorphic alleles, ssdp[neo48]/ssdp]BG1663] and ssdp[31]/ssdp[BG1663] were compared to each of the corresponding single heterozygotes 22 hybridizations were conducted, these include biological repeats and dye swaps as technical controls.

本研究针对两种不同ssdp减效等位基因的杂等位基因组合——ssdp[neo48]/ssdp[BG1663]与ssdp[31]/ssdp[BG1663]的三龄雄性幼虫开展转录谱分析，并将各组合的样本分别与对应的单杂合子样本进行比较。本次实验共完成22次杂交实验，其中包含生物学重复及作为技术对照的染料互换实验组。