Whole transcriptome analysis of endometroid cell lines after ERK5 inhibition

The transcriptomic network modulated after the inhibition of the nuclear tyrosin kinase ERK5 was analyzed Ishikawa endometroid cell line, in order to characterize and reveal any relevant role of this kinase at the transcriptional level. Ishikawa cells were treated with ERK5 inhibitor JWG-071 3µM during 24h. Later RNA was extracted in biological triplicates for RNA-Seq analysis.

本研究以Ishikawa子宫内膜样细胞系（Ishikawa endometroid cell line）为研究对象，分析了核酪氨酸激酶ERK5被抑制后所调控的转录组网络（transcriptomic network），旨在阐明并揭示该激酶在转录水平的相关功能。具体实验步骤如下：将Ishikawa细胞以浓度为3µM的ERK5抑制剂JWG-071处理24小时，随后完成三次生物学重复的RNA提取，用于RNA测序（RNA-Seq）分析。