Synchronization of growth and self-organizing digit pattern by WNT signaling [ChIPseq]

Molecular analysis of the spatio-temporal dynamics of destabilization and self-reorganization underscore the crucial role of WNT and BMP signaling modulators as part of the Turing system. Overall design: b-Catenin and H3K27Ac ChIP-seq embryonic forelimb buds 6 hours after DMSO or C59 injection at E10.5.

针对失稳过程与自重组现象的时空动态变化开展的分子分析，凸显了作为图灵系统（Turing system）组成部分的WNT与BMP信号调节剂所起到的关键作用。整体实验设计：于胚胎发育第10.5天（E10.5）向胚胎注射二甲基亚砜（DMSO）或C59，6小时后对胚胎前肢芽开展β-连环蛋白（β-Catenin）与组蛋白H3赖氨酸27乙酰化（H3K27Ac）的染色质免疫共沉淀测序（ChIP-seq）实验。