The_epidemiology_of_E__coli_and_non_typhoidal_Salmonella_at_a_household_level_in_Malawi__TENTACLE_

This is a longitudinal survey to investigate the transmission routes of E. coli and non-typhoidal Salmonella (NTS) at a household level in Malawi. Study sites will include randomly selected households in the Chikwawa district and Ndirande township, Blantyre, where faecal samples will be taken from humans, animals and the environment of each household. The main goals of the study are to determine the molecular epidemiology of E. coli, non-typhoidal Salmonella and antimicrobial resistance determinants within human and animal populations at a household level in Malawi, and drivers for transmission of these between individuals in each household. Using whole genome sequencing and the mSWEEP programmes we aim to develop candidate transmission routes of these bacteria and AMR determinants amongst humans, animals and the environment within each household.本研究为一项纵向调查，旨在探究马拉维地区家庭层面的大肠杆菌（E. coli）与非伤寒沙门氏菌（non-typhoidal Salmonella, NTS）的传播途径。研究现场涵盖布兰太尔市奇夸瓦区与恩迪兰德镇的随机抽选家庭，将从每户的人类、动物及其周边环境中采集粪便样本。本研究的主要目标为：明确马拉维家庭层面人类与动物群体中大肠杆菌、非伤寒沙门氏菌及抗菌药物耐药（Antimicrobial Resistance, AMR）决定因子的分子流行病学特征，同时解析各家庭内部个体间上述病原体的传播驱动因素。本研究将采用全基因组测序（whole genome sequencing）与mSWEEP分析程序，旨在构建各家庭内人类、动物与环境间上述细菌及AMR决定因子的潜在传播路径。