Dual RNA sequencing of PKD infected and uninfected brown trout (Salmo trutta) kidney samples

Proliferative kidney disease (PKD) in salmonid fish is caused by the myxozoan Tetracapsuloides bryosalmonae. We have performed dual RNA sequencing of the RNA collected from PKD infected and uninfected brown trout (Salmo trutta) kidneys. A total of 14 fish kidneys (8 infected and 6 uninfected fish) from three wild populations were selected. The goals for this study are: quantify the gene expression differences between the infected and uninfected fish; quantify the gene expression differences between the moderately and severely infected fish and de novo assemble the host-free parasite transcriptome.

鲑科鱼类增殖性肾病（Proliferative Kidney Disease, PKD）由黏孢子虫类的四联囊泡虫（Tetracapsuloides bryosalmonae）引发。本研究对感染PKD与未感染的褐鳟（Salmo trutta）肾脏组织的RNA开展了双转录组测序（dual RNA sequencing）。本次研究共选取了来自3个野生种群的14尾褐鳟肾脏样本，其中感染个体8尾、未感染个体6尾。本研究的目标如下：定量分析感染与未感染个体间的基因表达差异；定量分析中度感染与重度感染个体间的基因表达差异，并从头组装无宿主污染的寄生虫转录组。