Role of DNA methylation in stable gene repression

The experiment was designed to study the effects of the Dnmt1 gene knockout on gene expression, as well as to analyze the effects of TSA treatment on both WT (p53-/-) and knockout (p53-/-Dnmt1-/-) cells The effect of TSA treatment on knockout and WT cells was tested at two different time points (24 and 48 hs), as well as two weeks after removal of TSA Keywords: Effect of demetyhlation and/or reacetylation on gene expression Seven hybridizations were performed: 1. p53-/- untreated (control), 2. p53-/- +TSA harvested after 24hs of exposure to TSA, 3. p53-/- +TSA 48hs, 4. p53-/-Dnmt1-/- untreated, 5.p53-/-Dnmt1-/- +TSA 24hs 6.p53-/-Dnmt1-/- +TSA 48hs 7.p53-/-Dnmt1-/- two weeks after removal of TSA

本实验旨在探究Dnmt1基因敲除对基因表达的影响，并分析曲古抑菌素A（TSA）处理对野生型（WT，p53-/-）及敲除型（p53-/-Dnmt1-/-）细胞的作用。实验分别在两个不同时间点（24小时与48小时）对TSA处理后的两类细胞进行检测，同时还针对p53-/-Dnmt1-/-敲除型细胞检测了TSA撤除两周后的样本状态。关键词：去甲基化与/或重乙酰化对基因表达的影响。共完成7次杂交实验：1. 未处理的p53-/-细胞（对照组）；2. 经TSA处理24小时后收获的p53-/-+TSA细胞；3. 经TSA处理48小时的p53-/-+TSA细胞；4. 未处理的p53-/-Dnmt1-/-细胞；5. 经TSA处理24小时的p53-/-Dnmt1-/-细胞；6. 经TSA处理48小时的p53-/-Dnmt1-/-细胞；7. TSA撤除两周后的p53-/-Dnmt1-/-细胞。