RNA sequencing of human monocytes stimulated with murine platelets from wild-type or Clec1b-/- mice

C-type lectin receptors (CLRs) recognize glycans present on the surface of pathogens or self and mediate host defense as well as homeostasis. Dectin-1 is the most well-characterized CLR which recognizes β-glucan on various pathogens and expressed on human myeloid cells. Recently, we screened various tissue-derived primary cells for Dectin-1 binding and found that human Dectin-1 selectively binds to platelets. By establishing anti-platelet monoclonal antibodies that inhibit human Dectin-1 binding, we identified that CLEC-2 is responsible for the interaction with human Dectin-1. CLEC-2 is critical for lymphangiogenesis in early embryonic stage. In this study, we evaluated the influence on CLEC-2 binding in human monocytes which highly expresses human Dectin-1 by RNA sequencing. RNAseq analysis was performed by using RNA extracted from human monocytes stimulated with murine platelets from wild-type or Clec1b-/- mice.

C型凝集素受体（C-type lectin receptors, CLRs）可识别病原体或自身细胞表面的聚糖，介导宿主防御与内环境稳态。Dectin-1是目前研究最为透彻的CLR，可识别多种病原体表面的β-葡聚糖，且在人类髓系细胞中表达。近期，我们针对Dectin-1的结合特性筛选了多种组织来源的原代细胞，发现人类Dectin-1可选择性结合血小板。通过构建可抑制人类Dectin-1结合活性的抗血小板单克隆抗体，我们确定CLEC-2是介导与人类Dectin-1相互作用的受体。CLEC-2在胚胎早期的淋巴管生成过程中发挥关键作用。本研究中，我们采用RNA测序（RNAseq）技术，评估了高表达人类Dectin-1的人类单核细胞中CLEC-2结合活性所受的影响。本次RNAseq分析所用的RNA，提取自经野生型或Clec1b-/-小鼠的血小板刺激后的人类单核细胞。