CSDS抑郁模型小鼠海马体蛋白组学数据集

"尽管几十年的研究已经揭示了许多与抑郁症相关的海马体分子异常，但有关小鼠对慢性社会挫败压力(CSDS)诱导的抑郁症的易感性和恢复力的不同机制仍然知之甚少。通过社会失败模型，我们可以研究易感小鼠和适应性小鼠在分子变化上的差异。我们使用基于蛋白质组学的平台来比较CSDS小鼠和对照组小鼠的海马蛋白。通过等压标记相对和绝对定量(iTRAQ)结合LC-MS/MS鉴定差异表达蛋白。然后我们通过独创性途径分析(IPA)分析结果，并通过western blotting验证了5个蛋白。小鼠暴露于10天的CSDS，成功地诱导了应激易感和抗应激表型。与对照组相比，敏感组和弹性组分别有161和134个蛋白表达显著差异。主要有Rac信号通路和GABA受体信号通路。我们发现，与对照组相比，弹性小鼠低密度脂蛋白受体相关蛋白6 (LRP6)表达上调，易感小鼠神经肽Y (NPY)表达下调。与弹性组相比，敏感组小鼠神经肽Y受体2 (neuropeptide Y receptor type 2, NPY2R)蛋白表达下调。我们在这三组中的发现可能揭示了易感小鼠和适应性小鼠之间潜在的抑郁分子机制的差异。该结果为研究CSDS小鼠海马的分子异常以及治疗抑郁症的一些潜在药物靶点提供了新的思路。"

Although decades of research have uncovered numerous hippocampal molecular abnormalities associated with depression, the distinct mechanisms underlying susceptibility and resilience to chronic social defeat stress (CSDS)-induced depression in mice remain largely unknown. Using the social defeat model, we can investigate the differences in molecular alterations between stress-susceptible and stress-resilient mice. We employed a proteomics-based platform to compare hippocampal proteins between CSDS-exposed mice and control mice. Differentially expressed proteins were identified using isobaric tags for relative and absolute quantitation (iTRAQ) combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS). We then analyzed the results using Ingenuity Pathway Analysis (IPA) and validated five proteins via Western blotting. Mice exposed to 10 days of CSDS successfully developed stress-susceptible and stress-resilient phenotypes. Compared with the control group, 161 and 134 proteins were significantly differentially expressed in the susceptible and resilient groups, respectively. The key dysregulated pathways included the Rac signaling pathway and GABA receptor signaling pathway. We found that low-density lipoprotein receptor-related protein 6 (LRP6) was upregulated in resilient mice, while neuropeptide Y (NPY) was downregulated in susceptible mice compared with the control group. Compared with the resilient group, neuropeptide Y receptor type 2 (NPY2R) expression was downregulated in the susceptible group. Our findings across these three groups may reveal differences in the underlying molecular mechanisms of depression between susceptible and resilient mice. This study provides novel insights into the molecular abnormalities in the hippocampus of CSDS-exposed mice and potential therapeutic drug targets for depression.

搜集汇总

背景与挑战

背景概述

该数据集聚焦于慢性社会挫败压力（CSDS）诱导的小鼠抑郁模型，采用iTRAQ蛋白组学技术分析海马体组织中的蛋白表达差异。研究发现敏感组和弹性组分别有161和134个差异表达蛋白，涉及Rac信号通路和GABA受体信号通路，并识别了LRP6、NPY和NPY2R等关键分子，为揭示抑郁的分子机制和潜在药物靶点提供了新思路。