Expression data from the spinal cord of wild-type C57Bl/6 mice

The spinal cords of mice submitted to aortic cross-clamping for 7.5 minutes present with gray matter damage and central cord edema. 60% of mice subsequently experience hindlimb and tail paralysis. We used microarrays to detail the global programme of gene expression following aotic cross-clamping, in order to identify genes that could be responsible for edema and paralysis. RNAs extracted from the rostral (above T6) and caudal (below T6) part of the spinal cords of wild-type mice 24 and 48 hours following aortic cross-clamping were used for hybridization on Affymetrix microarrays. Mice submitted to sham surgery were used as controls.

接受7.5分钟主动脉交叉钳夹术的小鼠脊髓可出现灰质损伤及脊髓中央水肿，后续有60%的小鼠发生后肢与尾部瘫痪。本研究采用基因芯片（microarrays）对主动脉交叉钳夹术后的全局基因表达谱进行详细解析，以期识别出与水肿及瘫痪发病相关的基因。我们于主动脉交叉钳夹术后24小时及48小时，从野生型小鼠脊髓的头侧（T6节段以上）与尾侧（T6节段以下）区域提取RNA，将其用于阿菲梅特里克斯（Affymetrix）基因芯片的杂交实验。接受假手术的小鼠作为本研究的对照。