Quantitative Proteomic Profiling of Murine Embryonic Heart Development Reveals a Role for the Mevalonate Pathway in Cardiomyocyte Proliferation [Heart_RNAseq]

Purpose: The goals of this study are to characterize and analyze the emrbyonic heart transcriptome profiling (RNA-seq) at E9.5, E10.5, E12.5, E14.5 and E16.5. Methods: mRNA profiles of E9.5, E10.5, E12.5, E14.5 and E16.5 C57/Bl6 mouse embryonic hearts were generated by deep sequencing, n=3 or 4 for each age, using Illumina HiSeq2500. Results: Using an optimized data analysis workflow, we mapped at least 36 million sequence reads per sample to the mouse genome (build mm10) and identified 17,537 transcripts in the mouse hearts. E9.5. E10.5, E12.5, E14.5, E16.5 C57/Bl6 mouse embryonic hearts RNAseq

研究目的：本研究旨在对E9.5、E10.5、E12.5、E14.5及E16.5时期的小鼠胚胎心脏转录组进行RNA测序（RNA-seq）表征与分析。实验方法：采用Illumina HiSeq2500测序平台，对C57/BL6品系小鼠上述发育阶段的胚胎心脏开展深度测序以获取mRNA表达谱；每个发育阶段设置3~4个生物学重复（n=3或4）。研究结果：通过优化的数据分析流程，将每个样本至少3600万条序列读段比对至小鼠参考基因组（版本mm10），并在小鼠心脏样本中鉴定出17537个转录本。本数据集包含E9.5、E10.5、E12.5、E14.5及E16.5时期C57/BL6小鼠胚胎心脏的RNA测序数据。