Epigenetic landscape of Human Brains by Single Nucleus DNA Methylation and Chromatin Conformation Profiling - m3c29_h1930002_A24. Epigenetic landscape of Human Brains by Single Nucleus DNA Methylation and Chromatin Conformation Profiling - m3c29_h1930002_A24

Illustrating the cellular architecture of the human brain is critical to understanding its diverse functions and complex animal behaviors. Single nucleus methylation sequencing was applied to regions of human brains. we identified distinct cell clusters and formed them in a hierarchical taxonomy. These clusters include known primary brain cell types and possible sub-types. We use these data to identify the epigenomic characteristics and to define specific regulatory elements for each cell cluster. Overall design: Apply snm3C-seq to brain regions to identify epigenomic landcape of the adult human brains

阐明人类大脑的细胞架构，对于理解其多样功能与复杂动物行为至关重要。本研究对人类大脑多个脑区开展了单细胞核甲基化测序（Single nucleus methylation sequencing），成功识别出多个独特的细胞簇，并构建层级分类系统对其进行归类。这些细胞簇既包含已被报道的主要脑细胞类型，也涵盖潜在的细胞亚型。我们利用该数据集解析了各类细胞簇的表观基因组特征，并明确了每一类细胞簇特有的调控元件。实验整体设计：对脑区开展单细胞核3C测序（snm3C-seq），以解析成年人类大脑的表观基因组图谱。