Cross-reactions of anti-IspC MAbs with other L. monocytogenes serotypes.

aIsolate details can be found in Table S1.bThe interaction between each antibody-isolate pair was examined using ELISA in three independent experiments. Positive reactions were recorded if the average of the three OD414 measurements was >25% of the OD414 recorded for the same antibody when reacting with L. monocytogenes serotype 4b strain LI0521.cNegative reactions are reported if the average OD414 of the three independent experiments was 414 recorded for the same antibody when reacting with L. monocytogenes serotype 4b strain LI0521. In most cases a negative reaction could also be defined by OD414 of all three experiments being below the 25% threshold. However, M2777, M2781, M2792, M2795, M2797 and M2799 each frequently had one or two of three the measurements above the 25% threshold, even though the average remained below 25%. The variability of each of these MAbs makes them poor candidates for diagnostics.