Cross-reactions of anti-IspC MAbs with other L. monocytogenes serotypes.

aIsolate details can be found in Table S1.bThe interaction between each antibody-isolate pair was examined using ELISA in three independent experiments. Positive reactions were recorded if the average of the three OD414 measurements was >25% of the OD414 recorded for the same antibody when reacting with L. monocytogenes serotype 4b strain LI0521.cNegative reactions are reported if the average OD414 of the three independent experiments was 414 recorded for the same antibody when reacting with L. monocytogenes serotype 4b strain LI0521. In most cases a negative reaction could also be defined by OD414 of all three experiments being below the 25% threshold. However, M2777, M2781, M2792, M2795, M2797 and M2799 each frequently had one or two of three the measurements above the 25% threshold, even though the average remained below 25%. The variability of each of these MAbs makes them poor candidates for diagnostics.a. 分离株的详细信息参见表S1。 b. 采用酶联免疫吸附试验（ELISA）完成3次独立实验，检测每一组抗体-分离株配对的相互作用。当3次414nm光密度（OD₄₁₄）检测值的平均值，超出同一抗体与单核细胞增生李斯特菌（Listeria monocytogenes）血清型4b菌株LI0521反应时记录的OD₄₁₄值的25%时，判定为阳性反应。 c. 若3次独立实验的平均OD₄₁₄值低于上述同一抗体与单核细胞增生李斯特菌血清型4b菌株LI0521反应时记录的OD₄₁₄值的25%，则判定为阴性反应。多数情况下，阴性反应也可定义为3次实验的所有OD₄₁₄值均低于25%阈值。但M2777、M2781、M2792、M2795、M2797及M2799这6株单克隆抗体（Monoclonal Antibodies，MAbs）常出现3次检测中有1次或2次的OD₄₁₄值高于25%阈值的情况，即便其平均值仍低于该阈值。这类单克隆抗体的变异性较强，并非理想的诊断用候选试剂。