Analysis of nonsense-mediated mRNA decay in daf-2 mutants. Caenorhabditis elegans

To exmine the role of nonsense-mediated mRNA decay process in the longevity regulation of daf-2 mutants, we sequenced transcriptomes from day 1 adult Caenorhabditis elegans: Bristol N2 (wild-type), and smg-2(qd101), daf-2(e1370) and smg-2(qd101); daf-2(e1370) mutants. Overall design: Determination of transcript levels in N2 (wild-type), smg-2(qd101), daf-2(e1370), and smg-2(qd101); daf-2(e1370) animals by using deep sequencing, in duplicate

为探究无义介导的mRNA降解（nonsense-mediated mRNA decay, NMD）通路在daf-2突变体长寿调控中的作用，我们对第1天成年的秀丽隐杆线虫（Caenorhabditis elegans）转录组进行了测序，受试样本包括野生型 Bristol N2、smg-2(qd101)、daf-2(e1370) 以及 smg-2(qd101); daf-2(e1370) 双突变体。实验整体设计：采用深度测序技术对上述野生型及各突变体线虫的转录水平进行测定，每组设置两次平行重复。